Inflammatory Bowel Disease (IBD)
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- Inflammatory Bowel Disease (IBD)
Inflammatory Bowel Disease (IBD)
Inflammatory Bowel Disease (IBD), including ulcerative colitis and Crohn’s disease, arises from complex interactions between genetics, immune dysfunction, and the gut environment. Our IBD model portfolio includes both chemically induced and genetically engineered mouse models that replicate hallmark features of human IBD, such as mucosal inflammation, disrupted epithelial barriers, and dysregulated immune responses. These models are ideal for:
Investigating intestinal immune homeostasis
Studying cytokine and microbiota-driven inflammation.
Evaluating biologics, small molecules, probiotics, gene and cell therapies
Types of IBD Models
Model Name
DSS-induced Colitis
Type
Chemically Induced
Key Features
Acute ulcerative colitis or Chronic ulcerative colitis
Applications
Broad spectrum drug, Anti-inflammatory drug
Model Name
DNBS-induced Colitis
Type
Chemically Induced
Key Features
Acute Crohn’s disease Th1-driven colitis, transmural inflammation
Applications
Broad spectrum drug, immunotherapy evaluation
Model Name
B6-IL10 KO Mice
Type
Genetically Modified
Key Features
Spontaneous chronic colitis from dysregulated immune signalling
Applications
Probiotics study, Chronic IBD mechanisms, gut microbiota studies
Model Name
Anti-CD40-Induced Colitis
Type
Immune Activation Model
Key Features
T cell–dependent innate immune-driven colitis in immunodeficient mice
Applications
Biologics, Innate immunity, cytokine pathway investigation
DSS-induced Acute Colitis Model
Dextran sulfate sodium (DSS) is administered in drinking water to induce colonic epithelial damage and acute or chronic inflammation. This model mimics ulcerative colitis-like symptoms and is widely used for evaluating mucosal repair and anti-inflammatory therapies.
Key Features:
- Rapid onset of epithelial injury and inflammation
- Infiltration of innate immune cells, especially neutrophils
- Suitable for testing small molecules and biologics targeting mucosal healing
Cyclosporine A (CsA) alleviated weight loss and decreased disease activity index (DAI) score in UC mice. Pathological symptoms such as epithelial exfoliation of colon mucosa and inflammatory cell infiltration were relieved.
DSS-induced Chronic Colitis Model
The DSS-induced chronic colitis model is established by administering multiple cycles of DSS in drinking water, alternating with recovery periods. This induces relapsing and remitting inflammation in the colon, resembling human ulcerative colitis. The model is widely used to study chronic intestinal inflammation, epithelial damage, and therapeutic intervention over extended durations.
Key Features:
- Mimics chronic, relapsing-remitting ulcerative colitis
- Reproducible inflammation primarily in the distal colon
- Involves epithelial barrier disruption, immune cell infiltration, and mucosal ulceration
- Allows assessment of long-term efficacy and mucosal healing by drug candidates
- Easy to induce, cost-effective, and suitable for large-scale preclinical studies
Body weight-loss and DAI score increase were observed in DSS induced chronic colitis model.
DNBS-induced Colitis
2,4-Dinitrobenzene sulfonic acid (DNBS) is delivered rectally in ethanol, inducing Th1/Th17-mediated colitis and transmural inflammation. The model mimics several features of Crohn’s disease and is often used to assess immunomodulatory therapies.
Key Features:
- Strong Th1/Th17 immune response
- Transmural colonic inflammation and ulceration
- Mimics Crohn’s disease pathology
- Suitable for evaluating cytokine-targeting agents and immune suppressants
Treatment with 5-aminosalicylic acid reduced. The HE pathological score significantly, and pathological symptoms such as necrosis, exfoliation and inflammatory cell infiltration of colonic mucosa were relieved.
Anti-CD40 induced colitis model
In Rag-/- mice, a single injection of an agonistic anti-CD40 antibody induces rapid-onset colitis driven by innate immune activation. This T and B cell–independent model is used to explore innate immune mechanisms and test cytokine-targeted therapies.
Key Features:
- Rapid induction of innate-driven colitis
- No adaptive immune cells (T/B cell-deficient background)
- High levels of IL-23, TNF, and other innate cytokines
- Suitable for early-stage drug screening and innate pathway studies
H&E staining of colon demonstrating histopathological features associated with anti-CD40 induced colitis
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