Systemic Lupus Erythematosus (SLE) Models

Systematic Lupus Erythematosus (SLE) Models

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by T and B cell dysregulation, leading to inflammation and organ damage. The production of antinuclear antibodies (ANA) triggers immune complex deposition and complement activation, affecting the skin, kidneys, joints, and heart.

Model:
B6-Trex1-KO

Pathogenesis:
cGAS-STING-IFNα

Model:
B6-hBAFF

Pathogenesis:
B cell Activated

Model:
BALB/c-Tlr7-p.Y264H

Pathogenesis:
pDC activation related​

Model:
Pristane-Induced SLE

Pathogenesis:
Innate & Adaptive Immunity

Model:
TLR7 agonist Induced SLE in HSC-NCG-M

Pathogenesis:
Humanized Immunity

Model:
SLE patient derived PBMC induction

Pathogenesis:
Humanized Immunity

B6-Trex1-KO Mouse

B6-Trex1-KO Mouse

Strain: C57BL/6JGpt-Trex1em1Cd1194/Gpt
Strain ID: T013987

DESCRIPTION

The B6-Trex1-KO (knockout) mouse model lacks the Trex1 gene, which encodes a DNA exonuclease critical for preventing the accumulation of cytosolic DNA. Its deficiency leads to chronic activation of the type I interferon pathway and mimics autoimmune conditions such as Aicardi-Goutières syndrome (AGS) and systemic lupus erythematosus (SLE). This model is valuable for studying autoimmunity, innate immune sensing, and inflammation-related pathologies.

Survival Rate

Autoantibodies

Trex1-/- showed significantly increased Anti-dsDNA An

Organ Inflammation

Kidney: Inflammatory cell infiltration (black arrow); Bleeding (red arrow)

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B6-hBAFF

B6-hBAFF

Strain: C57BL/6JGpt-Tg(hBAFF)35/Gpt
Strain ID: T036794

DESCRIPTION

The B6-hBAFF mouse model expresses human B cell-activating factor (BAFF) on a C57BL/6 background. BAFF is essential for B cell survival, maturation, and function, and its dysregulation is associated with autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). This model is ideal for evaluating BAFF-targeted therapies and studying human B cell biology in vivo.

Anti-dsDNA Abs and Immunoglobulins

Increased anti-dsDNA and serum lg Levels in B6-hBAFF Mice

Lupus Nephritis

B6-hBAFF mice show Lupus Nephritis in 25 weeks

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Pristane induced SLE

Pristane induced SLE

Strain: BALB/c, C57BL/6, DBA

DESCRIPTION

The pristane-induced systemic lupus erythematosus (SLE) model is a chemically induced autoimmune model in which a single intraperitoneal injection of pristane (a hydrocarbon oil) in mice. triggers lupus-like symptoms. These include autoantibody production (e.g., anti-dsDNA), immune complex deposition, glomerulonephritis, and chronic inflammation. This model closely mimics aspects of human SLE pathogenesis and is widely used for studying disease mechanisms and evaluating potential immunomodulatory treatments.

Elevation of serum Anti-dsDNA post 9 weeks of pristane injection in BALB/c, C57BL/6 or DBA female mice

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Humanized SLE models

Humanized SLE Models

Strain: huHSC-NCG-M or NCG

APPLICATIONS
  • Evaluation of human-specific autoimmune responses such as Immune cell hyperactivity, autoantibody production, type I IFN signaling, cytokine and nephritis.
  • B-cell depletion therapies e.g CD19, CD20, BAFF antibodies and CAR-T / CAR-NK cell therapies
  • Interferon inhibitors
  • Immune checkpoint modulators1

Humanized SLE model construction

Key Features

Mostly T cells only

Study period 14-21 days

Anti-dsDNA increased from 2-4 weeks

Highly Donor dependent

Proteinuria

Relatively complete set of human immune system

TLR agonist (4-6 weeks) Pristane (18-24 weeks)

Lupus nephritis (Mild to moderate)

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