Multiple Sclerosis (MS) is a chronic autoimmune demyelinating disease of the central nervous system (CNS), characterized by immune-mediated destruction of myelin sheaths, neuroinflammation, and progressive neurodegeneration. The disease often presents with motor dysfunction, sensory impairment, and cognitive decline due to axonal injury and demyelination in the brain and spinal cord. Preclinical MS models are essential for studying disease mechanisms and evaluating potential therapies. The most widely used model is Experimental Autoimmune Encephalomyelitis (EAE), which can be induced in mice by immunization with either myelin-derived peptides such as MOG_35-55 for T-cell dependent EAE and MOG_1-125 peptide for B-cells dependent EAE.